摘 要： 目的调查贵阳市花溪区7～9岁小学生龋病流行情况，为制定针对性预防措施提供科学依据。方法按照《第三次全国口腔健康流行病学调查方案》的原则，采用整群抽样和分层抽样的方法，对花溪区的部分7～9岁小学生进行口腔健康检查，对结果进行统计学分析。结果检查的5759名儿童中，整体乳牙患龋率为63.1%，乳牙龋均为4.37；龋齿充填率为9.77%；第一磨牙萌出率为98.3%，患龋率为10.1%，而且随着年龄增加，第一磨牙患龋率明显升高。结论贵阳市花溪区儿童虽然乳牙患龋率低于全国平均水平，但龋齿充填率较低；第一磨牙患龋率高达10.1%，提示应加强对龋病的防治措施。
幽门螺杆菌(Helicobacter pylori，Hp)自1983年被Marshau和warren成功分离后，目前研究表明，Hp感染是世界上感染率最高细菌之 一，是慢性胃炎与十二指肠溃疡的致病因素.Hp感染已成为胃肠及胃肠外相关疾病研究的热点.世界卫生组织已将Hp确定为Ⅰ类致癌 原.广泛的调查显示我国的不同地区、不同民族的人群Hp检出率在30%～80%[2-5]，存在很大差异.本文于2006～2008年对贵阳市花溪区 1241名自然人群进行了Hp感染的流行病学调查，以探讨近年花溪区人群Hp感染的传染途径及感染危险因素.
Effects of Silencing of c- met Gene Expression on Biological Behavior of Gastric Cancer Cells With High Livermetastatic Potential
Background XGC9811- L，which is a gastric cancer cell line with high liver- metastatic potential，was established in the preliminary work of the study. The expression of c- met is higher in XGC9811- L than in other gastric cancer cell lines，and c- met is the receptor of hepatocyte growth factor. Objective To research the effects of silencing of c- met gene expression on biological behaviors of XGC9811- L，such as proliferation，invasion and metastasis. Methods From January to June 2014，using cell transfection method，we imported recombinant plasmid p SuppressorRetro- c- met- siRNA into XGC9811- L cells and built stable transfection cell lines. Based on whether transfection was made and the difference in the target genes of transfection，the stable transfection cell lines were divided into four groups： non- transfection group， empty carrier group，random sequence group and siRNA group. Fluorescent quantitation RT-PCR method was adopted to detect the relative expression level of c- met mRNA in XGC9811- L; c- met relative expression was measured by western blotting method. By cell proliferation assay，the cell proliferation ability of each group was detected，and cell growth curves were drawn. Invasion assay and movement experiment were conducted to calculate the number of transmembrane cells of each group. Results siRNA group was lower than the other three groups in the relative expression levels of c- met mRNA and c- met( P 0. 05). siRNA group was lower than the other three groups in cell proliferation ability on day 3，day 5，day 6 and day 7( P 0. 05). In invasion assay，siRNA group was less than the other three groups in the number of transmembrane cells( P 0. 05). In movement experiment，siRNA group was less than other three groups in the number of transmembrane cells( P 0. 05). Conclusion Silencing of c- met gene expression can inhibit the proliferation，invasion and metastasis of XGC9811- L，and c- met may play an important part in the occurrence and development of gastric hepatic metasis. The inhibition of c- met expression can inhibit the metastasis of gastric cancer cell to liver.
A case of xanthogranulomatous cholecystitis distinguished from hepatic invasion of gallbladder cancer using contrast-enhanced ultrasonography
A 49-year-old male was admitted with right upper quadrant pain. CT， US， and MRI showed abnormal thickening of gallbladder wall with multiple cystic components. The border between gallbladder wall and liver was unclear. We suspected xanthogranulomatous cholecystitis (XGC)， but cannot deny gallbladder carcinoma with hepatic invasion. To confirm tentative diagnosis， we performed contrast-enhanced US (CE-US). In the early phase of the CE-US， the gallbladder wall was homogenously enhanced， and all the cystic components were in the thickened wall. And then， in the later phase of the CE-US， the border between gallbladder wall and liver became clear. On the basis of these examinations， we diagnosed as XGC， rather than gallbladder carcinoma with hepatic invasion. Open cholecystectomy was performed and histopathological diagnosis was XGC.
摘 要： 目的：了解近年来花溪地区人群乙型肝炎病毒（HBV）表面抗原（HBsAg）携带情况，为预 防和控制HBV感染提供参考依据。方法：采用酶联免疫吸附试验（EIISA）法对本研究人群进行HBsAg检测并对结果作统计分析。结果：我院22785 体检人员中，HBsAg阳性1574人，阳性率6.12％，男性（7.66％）高于女性（4.15％），农村（9.97％）明显高于城镇（5.52％）和 学生（3.71％）。结论：花溪区人群HBsAg阳性率为6.12％，农村人群阳性率高达9.97％，明显高于城镇居民职工和学生。应加强农村人口乙肝相 关知识的健康宣教，进一步推进乙肝疫苗接种工作，提高农村人群的免疫力。
Tumor biomarkers: help or mislead in the diagnosis of xanthogranulomatous cholecystitis?-analysis of serum CA 19-9,carcinoembryonic antigen, and CA 12-5
BACKGROUND： (XGC) is a rare type of . Unlike other ， it can be easily misdiagnosed as based on radiological images. In response to misdiagnosis， extended surgical treatments are inappropriately given to patients， which is not beneficial to their health and/or recovery. In this study， we set out to determine whether biomarkers can help to avoid misdiagnosis in patients with XGC.： Between January 2005 and January 2012， a total of 37 preoperative patients at Sir Run Run Shaw Hospital were suspicious of having and was pathologically confirmed to be XGC after surgical operations. Before operations， all patients received a biomarker test to verify diagnosis， which included serum CA 19-9， ()， and CA 12-5.： A measured amount (54.05%) of cases (20 in 37) had at least one elevation over the thresholds of CA 19-9 (37 IU/L)， (5 ng/ml)， and CA 12-5 (35 IU/L)， which increased the suspicion of and consequently enhanced the difficulty to make right diagnosis of XGC as benign. 45.95% of cases (17 in 37) had an elevation in CA 19-9. 2.70% of cases (one in 37) had an elevation in and 24.32% of cases (nine in 37) had an elevation in CA 12-5. Analysis with Fisher's exact test discovered that the presence of common bile duct stone was a contributor to elevations of CA19-9 in patients with XGC. However， even in cases without common bile duct stones， 42.86% of patients (nine in 21) had elevations of at least one biomarker. Among them， 26.09% of patients (six in 21) had elevations of CA 19-9， with the maximum of 536.29 IU/L.： The elevations of biomarkers in XGC were frequent， suggesting their inabilities to clarify the disease's nature， especially when there was a suspicion of . Intraoperative frozen pathology of gallbladder might be a possible solution. However， it is against the en bloc surgical principle and has the potential to cause spreading. More research should be conducted， such as the discovery of a novel biomarker， so that XGC can less likely be misdiagnosed as until the final pathological judgment.
黄色肉芽肿性胆囊炎（Xanthogranulomatous cholecystitis，XGC）是一种慢性胆囊炎，胆囊内形成瘤样肿块的良性疾病，有破坏性，临床少见，易误诊胆囊癌或胆囊炎。本院自2000年 10月至2010年10月共收治23例，报道如下。
Abstract We reported a case of early cystic duct carcinoma concomitant with xanthogranulomatous cholecystitis (XGC). This case was a 72-year-old man in whom thickening of the gallbladder wall was pointed out an abdominal ultrasonography and elevation of the CA19-9 level was detected at a local clinic. Endoscopic ultrasonography and CT demonstrated a mass in the cystic duct. Mapping biopsy using peroral cholangioscopy (POCS) revealed a diagnosis of cystic carcinoma with superficial flat growth， therefore a pylorus-preserving pancreatoduodenectomy was performed. Histopathological diagnosis was well differentiated papillotubular adenocarcinoma with superficial flat spread and the thickening of the gallbladder wall was XGC. A case of early cystic duct carcinoma concomitant with XGC is extremely rare.
目的 探讨螺旋CT在黄色肉芽肿性胆囊炎(XGC)和厚壁型胆囊癌鉴别诊断中的价值.方法 回顾性分析18例XGC和20例厚壁型胆囊癌的CT征象，观察胆囊壁最大厚度、胆囊壁内的低密度结节、胆囊内壁黏膜线、增厚的胆囊壁的强化方式、是否合并 结石、胆囊周围邻近肝脏组织受累情况以及胆道梗阻情况.计量资料采用两组独立样本t检验进行分析，计数资料采用Fisher精确概率法进行比较.结果 XGC和厚壁型胆囊癌的平均最大胆囊壁厚度分别为(22.11±10.19) mm和(20.55±7.94)mm，差异无统计学意义(t=0.530，P=0.600)，XGC和厚壁型胆囊癌胆囊壁内有低密度结节者分别为18例和 5例(Fisher精确概率法，P＜0.01)，黏膜线完整的分别为14例和6例(Fisher精确概率法，P=0.004)，胆道梗阻分别为3例和12 例(Fisher精确概率法，P=0.009).CT征象中，胆囊壁厚度范围、增厚的胆囊壁的强化方式和程度、邻近肝脏浸润、淋巴结肿大、合并胆囊和 (或)胆管结石情况在XGC和厚壁型胆囊癌患者中均差异无统计学意义(Fisher精确概率法，P值均＞0.05).结论 增厚的胆囊壁内的低密度结节和较完整的胆囊内壁黏膜线是诊断XGC的特征性表现，螺旋CT扫描能为XGC和厚壁型胆囊癌的鉴别诊断提供依据.