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Therapeutic Effects Of PJW Ag 85B DNA Vaccine During Chemotherapy In Mice Infected With Mycobacterium Tuberculosis
Publication » Therapeutic Effects Of PJW Ag 85B DNA Vaccine During Chemotherapy In Mice Infected With Mycobacterium Tuberculosis.
design of 3 DNA vaccines， designated pJW4303-MEG1， pJW4303-MEG2 and pJW4303-MEG3， encoding multi-epitopes that are highly conserved within the HIV-1 subtypes most prevalent in China and can be recognized through HLA alleles dominant in China. The pJW4303-MEG1-encoded protein consisted of one Th epitope in Env， and one， 2， and 6 epitopes in Pol， Env， and Gag proteins， respectively， with a GGGS linker sequence between epitopes. The pJW4303-MEG2-encoded protein contained similar epitopes in a different order， but with the same linker as pJW4303-MEG1. The pJW4303-MEG3-encoded protein contained the same epitopes in the same order as that of pJW4303-MEG2， but with a different linker sequence (AAY). To evaluate immunogenicity， mice were immunized intramuscularly with these DNA vaccines. Both pJW4303-MEG1 and pJW4303-MEG2 vaccines induced equally potent humoral and cellular immune responses in the vaccinated mice， while pJW4303-MEG3 did not induce immune responses. These results indicate that both epitope and linker sequences are important in designing effective epitope-based vaccines against HIV-1 and other viruses.
AIMS： To describe and assess the efficacy of mechanical endonasal dacryocystorhinostomy (MENDCR). This is a new technique that involves creation of a large rhinostomy and mucosal flaps. The study involved a prospective non-randomised interventional case series with short perioperative follow up.： A prospective series of 104 consecutive endonasal DCRs performed from January 1999 to December 2001 were entered into the study. Patients included in the study had nasolacrimal duct obstruction and had not had previous lacrimal surgery. The technique involved anastomosis of nasal mucosal and lacrimal sac flaps and a large bony ostium. Surgery was performed by two surgeons (AT/PJW). Follow up assessment included nasoendoscopy as well as symptom evaluation. Success was defined as anatomical patency with flow on nasoendoscopy and patency to lacrimal syringing. The average follow up time was 9.7 months (range 2-28， SD 6.7 months).： There were 104 DCRs performed on 86 patients (30 male， 56 female). The average age of the patients was 59 years (range 3-89， SD 24.1 years). Common presentations were (77%) and/or (19%). Septoplasty (SMR) was required in 48 DCRs (46%) and 13 DCRs (12.5%) needed other endoscopic surgery in conjunction with the lacrimal surgery. The surgery was successful in 93 cases (89%). Of the 11 cases that were classified as a failure six patients was anatomically patent but still symptomatic and another two had preoperative canalicular problems. The anatomical patency with this new technique was thus 95% (99 of 104 DCRs).： MENDCR involves creation of a large ostium and mucosal preservation for the construction of flaps. The anatomical success is 95% and is similar to external DCR and better then other endonasal approaches. The authors suggest that creation of a large ostium as well as mucosal flaps improves the efficacy of this endonasal technique.
Development of an expression plasmid and its use in genetic manipulation of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (higher Basidiomycetes).
Abstract We report the construction of a plasmid， pJW-EXP， designed for the expression of homologous and heterologous genes in Ganoderma lucidum. pJW-EXP was generated from the plasmid pMD19-T by inserting the G. lucidum glyceraldehyde-3-phosphate dehydrogenase gene promoter， the G. lucidum iron-sulfur protein subunit of succinate dehydrogenase gene terminator and the homologous carboxin-resistance gene as selection marker. This expression plasmid can be efficiently transformed into Ganoderma through polyethylene glycol-mediated protoplast transformation. Southern blot analysis showed that most of the integrated DNA appeared as multiple copies in the genome. The applicability of the constructed plasmid was tested by expression of the truncated G. lucidum 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene that encodes the catalytic domain of HMGR. Overexpression of the truncated HMGR gene， which is a key gene in the biosynthetic pathway of the antitumor compounds， ganoderic acids， increased the transcription of the HMGR gene and enhanced ganoderic acid accumulation. pJW-EXP can serve as a useful tool in the genetic improvement and metabolic engineering of Ganoderma.
Construction and in Vitro Expression of Genetic Vaccine Encoding Hepatitis B Virus Core Antigen Fused to Tissue Plasminogen Activator Signal Sequence
Objective： To construct and express nucleic acid vaccine encoding hepatitis B virus core antigen(HBcAg) fused to tissue plasminogen activator signal sequence(tPA) in vitro.Methods： Restriction sites were introduced to HBcAg cDNA using polymerase chain reaction(PCR).The gene product was cloned to pJW4303 to construct nucleic acid vaccine and all constructs were identified by sequenc-ing，and 293T cells were transiently transfected with pJW4303/HBc/tPA，pJW4303/HBc and pJW4303 blank vector.The HBcAg protein was measured by western blot.Results： pJW4303/HBc/tPA nucleic acid vaccine was constructed successfully and expressed HBcAg in lysates and supernatants in 293t cells in vitro.Expression level of pJW4303/HBcAg/tPA was higher than that of pJW4303/HBc.Conclusion： HbcAg encoded by nucleic acid vaccine pJW4303/HBc/tPA and the expressed product in 293t cells could be secreted out of the cells.This research is the foundation for the further study of the pJW4303/HBc/tPA nucleic acid vaccine.
Vrenken H, Pouwels PJW, Geurts JJG, et al. Altered diffusion tensor in multiple sclerosis normal-appearing brain tissue: Cortical diffusion changes seem related to clinical deterioration. J Magn Reson Imaging 2006;23:628–636.
Comment on 'Bone SPECT reduces the number of unnecessary mandibular resections in patients with squamous cell carcinoma' [Van Can EM, Oyen WJG, Koole R, Stoelinga PJW. Oral Oncology 2006;42:409-14].
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本发明属于生物医药技术领域，涉及密码子优化的严重发热伴血小板减少综合征病毒核蛋白基因及其核酸疫苗。该密码子优化基因序列兼顾了哺乳动物细胞密码子使用偏好，序列如SEQ ID NO.2所示。所涉及的严重发热伴血小板减少综合征病毒核蛋白核酸疫苗由密码子优化的严重发热伴血小板减少综合征病毒核蛋白基因序列和真核表达载体pJW4303组成，其5’端连接tPA信号肽序列。该核酸疫苗在免疫哺乳动物后有效地刺激了免疫系统，产生了较好的体液免疫反应。
ABSTRACT Ecosystem engineering species， such as mussel， oysters， of salt-marsh vegetation， can attenuate wave energy to protect the sea wall. Here we demonstrate this idea using LEGO as surrogates for ecosystem engineering species. See the video also on youtube： https：//www.youtube.com/watch?v=IQx8BVK_PJw