目的了解宝应县居民主要死因，为制定防控措施提供依据。方法按国 际疾病分类(ICD-10)进行死因编码，按照卫生部(CCD-87)17大类整理分析。结果2014年宝应县报告死亡数6216人，粗死亡率6.85 译。其中男性3364例，粗死亡率7.76译；女性2852例，粗死亡率6.39译。2014年居民平均期望寿命为78.42岁，男性76.72岁，女性 80.16岁。主要死因顺位为恶性肿瘤、脑血管疾病、呼吸系统疾病、心脏病、损伤与中毒。结论慢性非传染性疾病(简称"慢性病")和伤害为宝应县居民的主 要死因，应大力开展健康教育及行为干预，改变不良的生活方式，增强安全意识，以达到有效降低居民死亡率的目的。
Abstract 4281: Oncostatin M renders epithelial cell adhesion molecule-positive liver cancer stem cells sensitive to 5-fluorouracil by inducing hepatocytic differentiation
Recent evidence suggests that a certain type of () is hierarchically organized by a subset of cells with stem cell features (stem cells; ). Although normal stem cells and are considered to share similar self-renewal programs， it remains unclear whether differentiation programs are also maintained in and effectively used for eradication. In this study， we investigated the effect of oncostatin M ()， an -related cytokine known to induce the differentiation of hepatoblasts into hepatocytes， on liver . receptor expression was detected in the majority of epithelial molecule-positive (EpCAM(+)) with stem/progenitor cell features. treatment resulted in the induction of hepatocytic differentiation of EpCAM(+) cells by inducing signal transducer and activator of 3 activation， as determined by a decrease in stemness-related gene expression， a decrease in EpCAM， alpha-fetoprotein and protein expressions， and an increase in albumin protein expression. -treated EpCAM(+) cells showed enhanced with expansion of the EpCAM-negative non-population. Noticeably， combination of treatment with the chemotherapeutic agent (5-FU)， which eradicates EpCAM-negative non-， dramatically increased the number of apoptotic cells in vitro and suppressed in vivo compared with either saline control， ， or 5-FU treatment alone. Taken together， our data suggest that could be effectively used for the differentiation and active of dormant EpCAM(+) liver ， and the combination of and conventional chemotherapy with 5-FU efficiently eliminates by targeting both and non-.
目的：对2009～2013年宝应县水痘流行趋势进行全面分析。 方法使用描述性流行病学法，以2009～2013年宝应县疾病预防控制中心434例水痘病例为研究对象，对其进行分析。结果2009～2013年宝应县水 痘发病人数为434人，无死亡案例，截止到2013年末，五年间水痘疾病平均发病率为0.048%。8月份发病人数最少，占总数的1.99%，12月份发 病人数最多，占总数的37.15%。整体趋势为发病人数随着年龄的增长而逐步增多，在5～14岁形成高峰之后随着年龄的增长出现逐年下降的趋势，呈现出单 峰分布。有1/4的病患在发病24 h内进行治疗。男性发病率高于女性，且相关数据存在统计学意义，P<0.05。结论在发病高峰期，做好宣传工作，医院方面做好治疗工作，强化对医护 人员的相关培训，能够减少该疾病误诊率，对疫情控制有非常重要的现实意义。
摘 要： 收集宝应县2005-2014年疟疾网络疫情报告数据和抗疟措施执行情况，描述性分析疟疾疫情及防治效果。宝应县2005-2014年共累计报告疟疾175例，无死亡病例。2005-2008年间日疟病例占86.4%（19/22），均为本地感染，发病集中于5-11月份。2009-2014年恶性疟病例占82.4%（126/153），均为境外感染，无明显的季节高峰。输入性病例呈上升趋势，从2007年的1例增至2009年的22例。2010-2014年报告的均为输入性病例。患者规范治疗率为100%，休止期根治195人，血检发热病人52 827人，疟原虫阳性率为0.3%（149/52 827）。宝应县自2010年以来无本地感染疟疾病例，但输入性疟疾形势依然需要重视。
Human aquaporin 4281-300 is the immunodominant linear determinant in the context of HLA-DRB1*03:01: relevance for diagnosing and monitoring patients with neuromyelitis optica.
Abstract OBJECTIVE To identify linear determinants of human aquaporin 4 (hAQP4) in the context of HLA-DRB1*03：01. DESIGN In this controlled study with humanized experimental animals， HLA-DRB1*03：01 transgenic mice were immunized with whole-protein hAQP4 emulsified in complete Freund adjuvant. To test T-cell responses， lymph node cells and splenocytes were cultured in vitro with synthetic peptides 20 amino acids long that overlap by 10 amino acids across the entirety of hAQP4. The frequency of interferon γ， interleukin (IL) 17， granulocyte-macrophage colony-stimulating factor， and IL-5-secreting CD4+ T cells was determined by the enzyme-linked immunosorbent sport assay. Quantitative immunofluorescence microscopy was performed to determine whether hAQP4281-300 inhibits the binding of anti-hAQP4 recombinant antibody to surface full-length hAQP4. SETTING Academic neuroimmunology laboratories. SUBJECTS Humanized HLA-DRB1*03：01+/+ H-2b-/- transgenic mice on a B10 background. RESULTS Peptide hAQP4281-300 generated a significantly (P <.01) greater TH1 and TH17 immune response than any of the other linear peptides screened. This 20mer peptide contains 2 dominant immunogenic 15mer peptides. hAQP4284-298 induced predominantly an IL-17 and granulocyte-macrophage colony-stimulating factor TH cell phenotype， whereas hAQP4285-299 resulted in a higher frequency of TH1 cells. hAQP4281-300 did not interfere with recombinant AQP4 autoantibody binding. CONCLUSIONS hAQP4281-330 is the dominant linear immunogenic determinant of hAQP4 in the context of HLA-DRB1*03：01. Within hAQP4281-330 are 2 dominant immunogenic determinants that induce differential TH phenotypes. hAQP4 determinants identified in this study can serve as diagnostic biomarkers in patients with neuromyelitis optica and may facilitate the monitoring of treatment responses to pharmacotherapies.
Abstract 4281: Determination of the Relative Potency of a Selective Agonist of the Intermediate-Affinity IL-2 Receptor on Lymphocytes from Human, Cynomolgus Monkey and Mouse
RDB 1450 is an engineered fusion protein designed to selectively activate the intermediate-affinity IL-2 receptor versus the high-affinity IL-2 receptor. The potencies of RDB 1450 and IL-2 for activation of distinct subsets of effector and regulatory lymphocytes from murine， non-human primate and human donors were determined. Splenocytes isolated from mice or leukocytes isolated from human or cyno blood were stimulated with either RDB 1450 or IL-2. Multicolor flow cytometry was used to identify distinct subpopulations and the extent of phosphorylation of STAT5 was measured. Whereas IL-2 is 2-3 orders of magnitude more potent on immunosuppressive T regs relative to NK cells and memory CD8 T cells， RDB 1450 induces activation of NK cells， memory CD8 T cells and T regs at comparable concentrations within each species. The non-differentiated potency of RDB 1450 on the lymphocyte subpopulations examined suggests that its effects are mediated through the intermediate affinity receptors even on CD25-expressing T regs . The preferential activation of T regs by IL-2 may lead to immunosuppression and limit its antitumor efficacy. In contrast， RDB 1450 does not exhibit the same preference for T reg activation and is expected to be more effective in driving antitumor immune responses. These results highlight the differentiated immunological profile of RDB 1450 and support its potential as a novel human immunotherapy.Citation Format： Emily E. Rosentrater， Heather Flick， Jared E. Lopes， Heather C. Losey， Chunhua Wang， Juan C. Alvarez. Determination of the Relative Potency of a Selective Agonist of the Intermediate-Affinity IL-2 Receptor on Lymphocytes from Human， Cynomolgus Monkey and Mouse. [abstract]. In： Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia， PA. Philadelphia (PA)： AACR; Cancer Res 2015;75(15 Suppl)：Abstract nr 4281. doi：10.1158/1538-7445.AM2015-4281
目的 了解宝应县农村生活饮用水水质状况，确保广大农民饮水安全.方法 按《生活饮用水标准检验方法》进行样品采集、运输及检测，按《生活饮用水卫生标准》、《地表水环境质量标准》进行评价.结果 全年共监测水样670份，合格样品564份，样品合格率为84.18％.监测总项次数为14 048项，合格项次数为13 908项，项次合格率为99.00％.主要不合格指标为微生物指标，菌落总数、总大肠菌群、耐热大肠菌群超标率分别为5.61％、2.88％、 2.73％.另外，二氧化氯和肉眼可见物的超标率也较高，分别为4.85％、2.73％.结论 尽管宝应县农村饮用水水质总体情况较好，各级政府及相关部门仍要重视生活饮用水安全问题，积极采取措施，确实保障广大农民身体健康.
Background： To provide a systematic analysis of formalin fixation artifacts on Illumina sequencing libraries and results， we generated two complementary sequencing libraries (target enrichment sequencing and whole exome sequencing) from 11 pairs of matched formalin-fixed paraffin-embedded (FFPE) and fresh-frozen (FF) tumor samples and two pairs of matched FFPE and FF germline samples. We also generated whole genome sequencing data from a single pair of FF/FFPE tumor samples. Results： The results indicate minimal variations in library fragment size， coverage， and PCR duplicates within FF/FFPE paired samples that are less than 1 year old; whereas， a large variation in these parameters were observed in FF/FFPE pairs in samples that are approximately 2 years old. No significant increase in global mismatch rates and C•G>T•A substitutions were observed in FFPE samples from the former group; whereas， a discernible increase in mismatch rates and C•G>T•A substitutions were observed in FFPE samples from the latter group. However， over 99.7% and 99.5% of concordant calls were observed between matched FF and FFPE pairs at reference and non-reference positions within the targeted regions， respectively. Although an increased rate of global mismatches and C•G>T•A substitutions were observed in some FFPE samples， discordant rates were low (T•A substitutions are comparable in non-reference positions in paired FF and FFPE samples. Conclusions： We developed upfront quality assessment and library preparation method that use low input DNA from FFPE samples to perform next-generation sequencing. The results from our studies indicate the suitability of FFPE samples in sequencing studies. Citation Format： Sarah Munchel， Yen Hoang， Yue Zhao， Joseph Cottrell， Brandy Klotzle， Andrew Godwin， Janelle Noel， Brooke Fridley， Peter Beyerlein， Jian-Bing Fan， Marina Bibikova， Jeremy R. Chien. Targeted or whole genome sequencing of formalin-fixed tissue samples. [abstract]. In： Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego， CA. Philadelphia (PA)： AACR; Cancer Res 2014;74(19 Suppl)：Abstract nr 4281. doi：10.1158/1538-7445.AM2014-4281