马凤楼教授是卫生学及营养学界又红又专的资深专家，对我国预防医学事业作出了杰出贡献.我与马教授相识于协和高师班，她在公共卫生科，我在生化科，迄今已 60多年，后来共同筹备中国营养学会复会及参加学会各种活动，使我对她的许多优秀品质有了进一步认识. 首先，她有坚强的党性，无论在地下工作时，或在新中国建设中，一切服从党和国家的需要.她的学习和工作多次调动，她都严于律己，克服个人工作和生活中的各 种困难，圆满完成了既定的任务.
正马凤楼教授生于1924年， 早年求学期间参加中共地下党。1948年创建江苏医学院第一个党支部，并任支部书记。1952年毕业后，为了响应发展我国预防医学事业的号召，她毅然放弃 了从事儿科的志愿，参加全国首届公共卫生高级师资班进修学习，从此一生为预防医学事业作出了重要贡献。马凤楼教授是我国著名的营养与食品卫生学专家，曾任 多届中国营养学会理事、常务理事;她也是中国营养学会老年营养分会的发起和创始人之一。她是江苏省营养学会的创建者并任第一届理事长，现任名誉理事长。曾 任江苏省预防医学会常务委员及食品卫生专业委员会主任委员、江苏省保健食品评审委员会副主任委员。她曾是《中国居民膳食指南》专家委员会委员、《中国居民 膳食营养素参考摄入量》修订专家委员会委员兼常量元素专家组副组长，《营养学报》、《中国食品卫生杂志》、《中国营养科学全书》等编委、顾问。曾荣获江苏 省优秀教育工作者称号，享受政府特殊津贴。
Abstract 4281: Oncostatin M renders epithelial cell adhesion molecule-positive liver cancer stem cells sensitive to 5-fluorouracil by inducing hepatocytic differentiation
Recent evidence suggests that a certain type of () is hierarchically organized by a subset of cells with stem cell features (stem cells; ). Although normal stem cells and are considered to share similar self-renewal programs， it remains unclear whether differentiation programs are also maintained in and effectively used for eradication. In this study， we investigated the effect of oncostatin M ()， an -related cytokine known to induce the differentiation of hepatoblasts into hepatocytes， on liver . receptor expression was detected in the majority of epithelial molecule-positive (EpCAM(+)) with stem/progenitor cell features. treatment resulted in the induction of hepatocytic differentiation of EpCAM(+) cells by inducing signal transducer and activator of 3 activation， as determined by a decrease in stemness-related gene expression， a decrease in EpCAM， alpha-fetoprotein and protein expressions， and an increase in albumin protein expression. -treated EpCAM(+) cells showed enhanced with expansion of the EpCAM-negative non-population. Noticeably， combination of treatment with the chemotherapeutic agent (5-FU)， which eradicates EpCAM-negative non-， dramatically increased the number of apoptotic cells in vitro and suppressed in vivo compared with either saline control， ， or 5-FU treatment alone. Taken together， our data suggest that could be effectively used for the differentiation and active of dormant EpCAM(+) liver ， and the combination of and conventional chemotherapy with 5-FU efficiently eliminates by targeting both and non-.
喜闻马凤楼老师90岁华诞，我回头一算，自从本科毕业离开母校已29年了，虽然时间久远，但至今还清晰地 记得1984年马老师在讲台上给我们授课的风采.大四那年我们主要学习卫生专业方面的专业课，马老师既教授我们营养与食品卫生学，还承担毒理学的授课.记 得马老师当时还担任学校的行政职务，同时也是全国营养与食品卫生方面的知名专家，工作繁忙，年龄也60出头了，还亲自登上讲台授课，对我们学生来说的确是 一件幸福的事.因为充分的备课和渊博的学识，马老师授课就如行云流水，深入浅出，易懂易记，很受学生们的欢迎和喜欢，也就是从那时起，我也喜欢上了营养 学，作为自己今后发展的专业.
Human aquaporin 4281-300 is the immunodominant linear determinant in the context of HLA-DRB1*03:01: relevance for diagnosing and monitoring patients with neuromyelitis optica.
Abstract OBJECTIVE To identify linear determinants of human aquaporin 4 (hAQP4) in the context of HLA-DRB1*03：01. DESIGN In this controlled study with humanized experimental animals， HLA-DRB1*03：01 transgenic mice were immunized with whole-protein hAQP4 emulsified in complete Freund adjuvant. To test T-cell responses， lymph node cells and splenocytes were cultured in vitro with synthetic peptides 20 amino acids long that overlap by 10 amino acids across the entirety of hAQP4. The frequency of interferon γ， interleukin (IL) 17， granulocyte-macrophage colony-stimulating factor， and IL-5-secreting CD4+ T cells was determined by the enzyme-linked immunosorbent sport assay. Quantitative immunofluorescence microscopy was performed to determine whether hAQP4281-300 inhibits the binding of anti-hAQP4 recombinant antibody to surface full-length hAQP4. SETTING Academic neuroimmunology laboratories. SUBJECTS Humanized HLA-DRB1*03：01+/+ H-2b-/- transgenic mice on a B10 background. RESULTS Peptide hAQP4281-300 generated a significantly (P <.01) greater TH1 and TH17 immune response than any of the other linear peptides screened. This 20mer peptide contains 2 dominant immunogenic 15mer peptides. hAQP4284-298 induced predominantly an IL-17 and granulocyte-macrophage colony-stimulating factor TH cell phenotype， whereas hAQP4285-299 resulted in a higher frequency of TH1 cells. hAQP4281-300 did not interfere with recombinant AQP4 autoantibody binding. CONCLUSIONS hAQP4281-330 is the dominant linear immunogenic determinant of hAQP4 in the context of HLA-DRB1*03：01. Within hAQP4281-330 are 2 dominant immunogenic determinants that induce differential TH phenotypes. hAQP4 determinants identified in this study can serve as diagnostic biomarkers in patients with neuromyelitis optica and may facilitate the monitoring of treatment responses to pharmacotherapies.
摘 要： 今年正值马凤楼教授诞辰90周年，回顾马教授90年的人生历程，她的大部分人生与南京医科大学公共卫生学院的发展，尤其是营养与食品卫生学科的飞速前进紧密相联。
Abstract 4281: Determination of the Relative Potency of a Selective Agonist of the Intermediate-Affinity IL-2 Receptor on Lymphocytes from Human, Cynomolgus Monkey and Mouse
RDB 1450 is an engineered fusion protein designed to selectively activate the intermediate-affinity IL-2 receptor versus the high-affinity IL-2 receptor. The potencies of RDB 1450 and IL-2 for activation of distinct subsets of effector and regulatory lymphocytes from murine， non-human primate and human donors were determined. Splenocytes isolated from mice or leukocytes isolated from human or cyno blood were stimulated with either RDB 1450 or IL-2. Multicolor flow cytometry was used to identify distinct subpopulations and the extent of phosphorylation of STAT5 was measured. Whereas IL-2 is 2-3 orders of magnitude more potent on immunosuppressive T regs relative to NK cells and memory CD8 T cells， RDB 1450 induces activation of NK cells， memory CD8 T cells and T regs at comparable concentrations within each species. The non-differentiated potency of RDB 1450 on the lymphocyte subpopulations examined suggests that its effects are mediated through the intermediate affinity receptors even on CD25-expressing T regs . The preferential activation of T regs by IL-2 may lead to immunosuppression and limit its antitumor efficacy. In contrast， RDB 1450 does not exhibit the same preference for T reg activation and is expected to be more effective in driving antitumor immune responses. These results highlight the differentiated immunological profile of RDB 1450 and support its potential as a novel human immunotherapy.Citation Format： Emily E. Rosentrater， Heather Flick， Jared E. Lopes， Heather C. Losey， Chunhua Wang， Juan C. Alvarez. Determination of the Relative Potency of a Selective Agonist of the Intermediate-Affinity IL-2 Receptor on Lymphocytes from Human， Cynomolgus Monkey and Mouse. [abstract]. In： Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia， PA. Philadelphia (PA)： AACR; Cancer Res 2015;75(15 Suppl)：Abstract nr 4281. doi：10.1158/1538-7445.AM2015-4281
Background： To provide a systematic analysis of formalin fixation artifacts on Illumina sequencing libraries and results， we generated two complementary sequencing libraries (target enrichment sequencing and whole exome sequencing) from 11 pairs of matched formalin-fixed paraffin-embedded (FFPE) and fresh-frozen (FF) tumor samples and two pairs of matched FFPE and FF germline samples. We also generated whole genome sequencing data from a single pair of FF/FFPE tumor samples. Results： The results indicate minimal variations in library fragment size， coverage， and PCR duplicates within FF/FFPE paired samples that are less than 1 year old; whereas， a large variation in these parameters were observed in FF/FFPE pairs in samples that are approximately 2 years old. No significant increase in global mismatch rates and C•G>T•A substitutions were observed in FFPE samples from the former group; whereas， a discernible increase in mismatch rates and C•G>T•A substitutions were observed in FFPE samples from the latter group. However， over 99.7% and 99.5% of concordant calls were observed between matched FF and FFPE pairs at reference and non-reference positions within the targeted regions， respectively. Although an increased rate of global mismatches and C•G>T•A substitutions were observed in some FFPE samples， discordant rates were low (T•A substitutions are comparable in non-reference positions in paired FF and FFPE samples. Conclusions： We developed upfront quality assessment and library preparation method that use low input DNA from FFPE samples to perform next-generation sequencing. The results from our studies indicate the suitability of FFPE samples in sequencing studies. Citation Format： Sarah Munchel， Yen Hoang， Yue Zhao， Joseph Cottrell， Brandy Klotzle， Andrew Godwin， Janelle Noel， Brooke Fridley， Peter Beyerlein， Jian-Bing Fan， Marina Bibikova， Jeremy R. Chien. Targeted or whole genome sequencing of formalin-fixed tissue samples. [abstract]. In： Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego， CA. Philadelphia (PA)： AACR; Cancer Res 2014;74(19 Suppl)：Abstract nr 4281. doi：10.1158/1538-7445.AM2014-4281
正2015年1月31日上午 9：00，在欢乐祥和的气氛中，中国医疗保健国际交流促进会第五次全国会员代表大会在北京瑞安宾馆拉开帷幕，历时1天。参会代表由第四届主要领导、换届筹 备领导小组成员、所属分支机构的主要负责人、专家及推选的代表构成。民政部民间组织管理局孙伟林局长，原中央纪委驻卫生部纪检组长、卫生部原副部长、中国 保健协会张凤楼会长，原卫生部副部长孙隆椿，原卫生部副部长、我会原名誉会长曹泽毅等
The ubiquitin ligase Nedd4 is an ubuiquitin ligase (E3) which belongs to the HECT family. Like other member of this family members， such as Itch and Smurf， Nedd4 has been proposed to regulate a number of signaling pathways， as well as the traffic of several cell surface molecules， however， its physiological role in mammals has not been well characterized. Former reports identified reduction in the mitogenic activity and increase of the adaptor protein Grb10， aberrant IGF-1， VEGF and FGF receptor localization and / or signaling. In the present study， we performed an analysis of Nedd4-null murine embryonal fibroblastoid cells (MEFs) to show that loss of Nedd4 may affect any additional growth factor signaling. Surface expression of PDGF receptor was significantly increased in homozygous mutant mouse embryonic fibroblasts. Accordingly， PDGF-BB stimulation induced enhanced signaling as judged by MAP kinase p42/44 and Akt phosphorylation. Knockdown of Nedd4 in cell lines also induced similar phenotype. Nedd4， when transiently expressed， ubiquitinated PDGF receptor， which lead to degradation. Under the presence of lysosome inhibitors， PDGF receptor down regulation was at least partially blocked， suggesting its lysosome-dependent degradation. Subcellular localization of the PDGF receptor was also analyzed， but apparent difference was not observed. In the scratch assay， Nedd4 knockout cells showed enhanced migration activity， suggesting their active movement. Thus， Nedd4 appears to negatively control PDGF signaling partly through the regulation of its lysosome dependent degradation.Citation Format： Nobuyuki Tanaka， Kyoko Oyama， Keiichi Tamai. Nedd4 controls cell migration by regulating PDGF receptor signaling. [abstract]. In： Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington， DC. Philadelphia (PA)： AACR; Cancer Res 2013;73(8 Suppl)：Abstract nr 4281. doi：10.1158/1538-7445.AM2013-4281